TABLE 7.
Optimal bead mill homogenization conditions for obtaining maximum DNA yield and/or maximum DNA fragment size
| Homogenizer | Extraction method | Homogenization conditions
|
DNA characteristicsa
|
Purification method | ||
|---|---|---|---|---|---|---|
| Speed (rpm) | Time (s) | Yield | DNA fragment size | |||
| Mini Bead Beater | Procedure 3 | 3,300 | 120 | Best | Best | Sephadex G-200 |
| Mini Bead Beater-8 | Procedure 3 | 2,510 | 30 | OK | Best | Sephadex G-200 |
| Mini Bead Beater-8 | Procedure 3 | 2,510 | 120 | Best | OK | Sephadex G-200 |
a
DNA was characterized on the basis of total yield and size of the largest DNA fragments. Best indicates either maximum yield or maximum DNA fragment size compared to all other conditions tested. OK indicates adequate but not maximal DNA yield or fragment size.