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. 2022 May 18;25:335–349. doi: 10.1016/j.omto.2022.05.003

Figure 2.

Figure 2

AWARI CAR-T cells maintain fitness at resting state

(A) Diagram of the procedure used to investigate CAR-T cell products fitness. The analyses were performed on T cells transduced at similar efficiency. %CAR+: ARI = 27.0% ± 3.8% and AWARI = 23.4% ± 3.7%; VCN: ARI = 3.1 ± 1.1 vcn/c and AWARI = 2.9 ± 1.1. (B) Left: representative histograms show phospho-CD3z (p-CD3z) expression of NT (gray), ARI (blue), and AWARI (green) CAR-T cells. Right: graphical representation of pCD3z MeFI levels of NT (blue), ARI (red), and AWARI (green) CAR-T cells is shown. Two-tailed paired t test is shown (four independent donors; n ≥ 5). ∗p < 0.05. (C) Graph shows Fas (CD95) MeFI levels of NT (blue), ARI (red), and AWARI (green) CAR-T cells. Two-tailed paired t test (n = 4 and 3 independent donors). ∗∗p < 0.01. (D) Top: representative histograms of LAG-3, PD1, and Tim3 expression of NT (gray), ARI (blue), and AWARI (green) CAR-T cells are shown. Bottom: fold change of LAG-3, PD-1, and Tim3 expression levels of ARI (red) and AWARI (green) CAR-T cells related to the expression in NT cells (blue) is shown. Data are MeFI ± SEM. Two-tailed paired t test is shown (four independent donors; n ≥ 5), ∗p < 0.05.