Table 4.
Key data available for the G3 RHINESSA adult offspring (18+ years), and their G1 grandparents, G2 parents and G4 offspring. For the G2 generation, information from three study waves are presented (x available for all; ss available in subsample– see footnotes)
| Grandparents (G1) born 1898–1965 | Parents (G2) at 20–44 years RHINE/ECRHS I |
Parents (G2) at 30–54 years RHINE/ECRHS II |
Parents (G2) at 40–64 years RHINE/ECRHS III |
RHINESSA (G3) adult offspring 18–53 years* |
Offspring’s (G4) offspring age 0–30 years | |
| Questionnaire/interview | ||||||
| Social class, education | x | x | x | x | x | ss |
| Childhood factors | x | x | x | x | x | ss |
| Birth characteristics† | ss | ss | ||||
| Place of upbringing | x | x | x | x | x | ss |
| Smoking | x | x | x | x | x | ss |
| Snus, oral moist tobacco, E-cigarettes | x | ss | ||||
| Occupation | ss | x | x | x | x | ss |
| Indoor environment | ss | x | x | x | x | ss |
| Personal care products | ss | x | x | ss | ||
| Height/weight | ss | x | x | x | x | ss |
| Body shapes | x | x | x | ss | ||
| Waist circumference (self-measured) | x | x | ||||
| Physical activity | ss | ss | x | x | x | ss |
| Diet | ss | ss | ss | x | x | ss |
| Allergic diseases/symptoms | x | x | x | x | x | x |
| Asthma/symptoms | x | x | x | x | x | x |
| Asthma/allergy treatment | ss | x | x | x | x | ss |
| Sleep | ss | ss | ss | x | x | ss |
| Other diseases/symptoms | x | x | x | x | x | ss |
| Quality of life SF-36/RAND | ss | ss | ss | ss | ss | |
| Work disability | ss | x | x | |||
| Air pollution and greenness‡ | ss | ss | ||||
| Women questionnaire/interview (from women in each cohort) | ||||||
| Pregnancies and complications | x | x | x | ss | ||
| Birth characteristics of offspring | x | x | x | ss | ||
| Menarche, menstrual data, menopause | x | x | x | ss | ||
| Exogeneous sex hormones | x | x | x | ss | ||
| Irregular menstruation, PCOS | x | x | x | ss | ||
| Gynaecological and related diseases | x | x | x | ss | ||
| Clinical measures (from clinical stage in each cohort) | ||||||
| Anthropometry (height/weight/waist/hip) | ss | x | x | x | x | ss |
| Bioimpedance | ss | x | ss | ss | ||
| Spirometry (FEV1, FVC) | ss | x | x | x | x | ss |
| Post-BD spirometry | ss | x | x | ss | ||
| Metacholine test | x | x | ||||
| FeNO | ss | x | x | ss | ||
| Skin prick test | ss | x | x | x | ss | |
| Blood pressure | ss | x | x | ss | ||
| Heart rate | ss | x | x | ss | ||
| CIMT (carotis intima media) | ss | |||||
| CPI/caries index | ss | ss | ||||
| Biological material and environmental samples (from clinical stage in each cohort) | ||||||
| Blood samples | ss | x | x | x | x | ss |
| Gingival samples | ss | ss | ss | ss | ||
| Skin swab | ss | ss | ss | |||
| Saliva | ss | ss | ss | |||
| Urine | ss | ss | x | x | ss | |
| Bedroom dust samples | ss | ss | ss | ss | ss | |
| Biomarkers measured/funded at time of publication (from clinical stage in each cohort) | ||||||
| Total and specific IgEs | ss | x | x | x | x | ss |
| Genome wide genotyping | ss | ss | ||||
| Selective genotyping | ss | ss | ||||
| DNA methylation in fullblood | ss | ss | ss | ss | ss | |
| Fasting blood glucose | ss | ss | ss | ss | ||
| Sex hormones | women | women | women | ss | ss | |
| Oral microbiome | ss | ss | ||||
| Urine biomarkers of chemical exposures | ss | |||||
| Complete blood cell counts | ss | |||||
| Adipokines | ss | ss | ||||
Subsamples.
Grandparents and offsprings’ offspring were only investigated in Bergen, information in other centres are given by family members.
CIMT and CPI were only measured in Bergen.
Gingival samples were collected in parents and offspring from Bergen, Melbourne and Tartu, and in offspring from Uppsala.
DNA methylation was measured in fullblood using the Illumina EPIC BeadChip arrays in approximately 900 offspring, 400 parents and 140 grandparents.
Sex hormones were measured in mothers and approx. 1000 offspring from all centres.
Oral microbiome was measured using 16S rRNA Illumina MiSeq in Bergen adult/adolescent offspring and grandparent.
Urine biomarker concentrations of chemical exposure was measured in Bergen adult/adolescent offspring.
Complete blood cell counts were measured in Swedish centres, adipokines also in Reykjavik.
Helminth serology was measured in offspring from Bergen, Tartu and Aarhus, and parents from Bergen.
*Sweden and Iceland did a shorter clinical protocol of RHINESSA adult offspring, not including bioimpedance, skin swap or saliva (except that Uppsala collected saliva). RHINESSA offspring <18 years were included in Aarhus, Bergen, Melbourne and Tartu, following age-adapted slightly shorter protocols, similar to protocols used for corresponding age groups in offspring’s offspring.
†Information from registries and hospital protocols, and from mothers.
‡Information generated using geocoding based on registry data on life-time addresses.
BD, bronchodilator; CPI, Community Periodontal Index; ECRHS, European Community Respiratory Heath Survey; FeNO, Fractional exhaled nitric oxide; FEV1, Forced Expiratory Volume in 1 second; FVC, Forced Vital Capacity; PCOS, Polycystic ovary syndrome; RHINE, Respiratory Health In Northern Europe; RHINESSA, Respiratory Health in Northern Europe, Spain and Australia; SF-36, Short Form 36 Health Survey Questionnaire.