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. 2022 Mar 15;71(6):1170–1181. doi: 10.2337/db21-0706

Figure 3.

Figure 3

Maternal exercise-induced H3K4me3 stabilization is mediated through protection against WDR82 carbonylation. A: DNP-labeled carbonylated proteins in livers of offspring of chow- or HFD-fed and sedentary (Sed) or trained dams. B: WDR82 protein expression in livers of offspring of chow- or HFD-fed and sedentary or trained dams. C: DNP immunoblotting of WDR82-immunoprecipitated proteins in livers of offspring of chow- or HDS-fed and sedentary or trained dams (n = 3). **P < 0.001 vs. Chow-Sed; ****P < 0.0001 vs. Chow-Sed; ††P < 0.001 High Fat-Sed vs. High Fat-Train. Effects of Wdr82 overexpression on H3K4me3 levels (D), H3K4 methyltransferase activity (E), and mRNA expression of glucose metabolism genes (F) in primary hepatoblasts of chow- or HFD-fed dams (n = 3). *P < 0.025 vs. Chow-control (Con) (Wdr82−); **P < 0.01 vs. Chow-Con (Wdr82−); ***P < 0.001 vs. Chow-Con (Wdr82−); ****P < 0.0001 vs. Chow-Con (Wdr82−); †P < 0.025 High Fat-Wdr82− vs. High Fat-Wdr82 overexpression (Wdr81+); ‡P < 0.01 High Fat-Con (Wdr82−) vs. High Fat-Wdr82+; ††P < 0.001 High Fat-Wdr82− vs. High Fat-Wdr82+). Effects of Wdr82 knockdown on H3K4me3 levels (G) and mRNA expression of glucose metabolism genes (H) in primary hepatoblasts (n = 3). All data are reported as means ± SEM. **P < 0.001; ***P < 0.001. Statistical significance was determined by one- or two-way ANOVA, with Tukey and Bonferroni post hoc analysis.