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. 2022 Mar 15;71(6):1170–1181. doi: 10.2337/db21-0706

Figure 5.

Figure 5

Effects of SOD3 on offspring glucose metabolism are distinct from NAC. A and D: Developmental system used to treat offspring livers with recombinant SOD3 or NAC exo utero. Offspring livers were collected at 4 weeks (A) or at E13.5 (D). Glucose production in primary hepatocytes of 4-week-old male (B) and female (C) offspring of HFD-fed, saline-, SOD3-, or diethyldithiocarbamate (DETCA)-treated dams (n = 3). **P < 0.01 vs. pCPT-saline. Effects of SOD3 or NAC treatment in utero on ROS levels (E), carbonylated protein content (F), WDR82 carbonylation levels (G), mRNA expression of glucose metabolism genes (H), AMPKα phosphorylation (pAMPKα) levels (I), and mRNA expression of Tet and Idh (J) in livers of E13.5 offspring of HFD-fed dams (n = 3). IP, immunoprecipitation. All data are reported as means ± SEM. **P < 0.01 vs. pCPT-saline; ***P < 0.01 vs. pCPT-saline; ****P < 0.01 vs. pCPT-saline. Statistical significance was determined by one- or two-way ANOVA, with Tukey and Bonferroni post hoc analysis.