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. 2022 Apr 4;71(6):1246–1260. doi: 10.2337/db21-0681

Figure 3.

Figure 3

Glucose stimulation augments DOC2b tyrosine phosphorylation and interaction with YES kinase in MIN6 β-cells. A, left: Representative Western blot images of DOC2b-GFPWT–transfected MIN6 β-cells treated with either 20 mmol/L glucose for 2 min or 50 mmol/L KCl for 1 min followed by IP and immunoblot (IB). A, right: Quantification of the IBs from three independent experiments. B, left: Representative Western blot images of MIN6 β-cells transfected with rDOC2b-GFPWT plasmid and stimulated with 20 mmol/L glucose (2 min) with or without 20 μmol/L SU6656 pretreatment (2 h) followed by IP and IB analysis. B, right: Quantification of the IBs from three independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.002; ***P < 0.0002.