Figure 4.

YES kinase knockdown ablates tyrosine phosphorylation of DOC2b in MIN6 β-cells. Representative Western blot images of MIN6 β-cells transfected with rDOC2b-GFP^WT plasmid along with control (siControl) or siYES oligonucleotides. After a 48-h incubation, the cells were preincubated in modified Krebs-Ringer bicarbonate buffer for 2 h and stimulated with 20 mmol/L glucose (2 min) followed by IP and immunoblot (IB) analysis. Vertical dashed lines denote splicing of lanes from within the same gel exposure. YES/tubulin ratios demonstrate ∼40% siYES-induced knockdown efficiency. Bar graph quantification of the IBs from six independent experiments. Data are shown as mean ± SEM. *P < 0.05; **P < 0.002.