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. 2022 May 5;298(6):102014. doi: 10.1016/j.jbc.2022.102014

Figure 1.

Figure 1

Tau aggregation initiates in the cytoplasm.A, diagram of constructs used for this study. Expression of full-length (FL) tau was regulated by a tetracycline response element (TRE). B, U2OS cells expressing FL tau-Clo containing the P301S mutation and stained for tubulin, without exogenous wildtype fibril addition. FL tau-Clo colocalizes with tubulin. C, with exogenous fibril addition, tau redistributes to inclusions in the cytoplasm. D, recombinant FL tau fibrils labeled with AF647 (red) were applied to the U2OS cells expressing FL tau-Clo (green) and imaged over time to track tau aggregation (labeled). Note rapid and simultaneous development of tau inclusions throughout the cytoplasm. Scale bars = 10 μm. AF647, Alexa Fluor 647.