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. 2022 Apr 30;298(6):102004. doi: 10.1016/j.jbc.2022.102004

Figure 11.

Figure 11

TMPRSS2 knockdown in H441 human airway epithelial cells does not affect normal epithelial monolayer formation in culture.A, left panel, representative western blot showing endogenous expression of TMPRSS2 in H441 cells without (wildtype) or with TMPRSS2 knockdown (models 1 and 2) detected using a TMPRSS2-specific antibody. TMPRSS2 in its activated cleaved form (catalytic chain, ∼24 kDa) is indicated by a filled arrowhead. Right panel, densitometric evaluation of TMPRSS2 expression from similar blots as shown in left panel. In each blot, the density value of the ∼24 kDa TMPRSS2 band obtained for TMPRSS2-knockdown model 1 (n = 13) or model 2 (n = 9) was normalized to that of the corresponding TMPRSS2 band obtained in wildtype. Dashed line indicates a normalized density value of one (no effect). Mean ± SD and individual data points are shown; ∗∗∗p < 0.001; one-sample Student’s t test compared with wildtype (1.0). B, transepithelial electrical resistance (TEER) values recorded in wildtype or two different TMPRSS2-knockdown H441 cell models on day 8 after seeding cells on permeable supports. Mean ± SD and individual data points are shown; ns, one-way ANOVA with Bonferroni post hoc test (49 ≤ n ≤ 56). C, immunofluorescence staining for the tight junction Zonula occludens-1 protein (ZO-1, in green) in control or two different TMPRSS2-knockdown H441 cell models was performed on day 9 after seeding cells on permeable supports. One representative image is shown (n = 3). ns, not significant; TMPRSS2, transmembrane serine protease 2.