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. 2022 May 20;10:879428. doi: 10.3389/fcell.2022.879428

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Copyright © 2022 Wen, Lin, Wu, Cao and Fu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Zfp281 regulates the 2C-like transition of mESCs. (A) The sgRNA count enrichment from the published negative CRISPR-Cas9 genomic screen (Fu et al., 2019). Zfp281 is one of the top candidates inhibiting the 2C-like transition in the screen. (B) Western blot confirming efficient Zfp281 (bottom band) perturbation. sgZfp281#1 and sgZfp281#2 cause 67 and 38% decrease of Zfp281 expression respectively. (C) Relative Zscan4d mRNA levels normalized to Gapdh in Dux-activated mESCs. (D) The percentage of 2C-like cells after 24-hour Dux induction of the indicated manipulation in mESCs by FACS. (B–D) The X in sgX refers to the gene that sgRNA targets to; sgSv40 is negative control. Shown are mean ± s.d, p Values were calculated by unpaired t-test, two-tailed, two-sample unequal variance, *** <0.001.