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. 2022 May 20;10:879428. doi: 10.3389/fcell.2022.879428

FIGURE 3.

FIGURE 3

Zfp281 represses the 2C-like transition through Tet1. (A) Average occupancy plots and heatmaps of Tet1 signal within 5 kb of the center of TSS (transcription start sites) region of the Zfp281-bound-2C-upregulated genes. (B–D) Relative mRNA levels of Zfp352 (B), MERVL (C), and Zscan4d (D) normalized to Gadph in mESCs upon indicated manipulation. Zfp281 majorly impedes the pluripotent-to-2C-like state transition (Figure 2). To accurately analyze the effect of Zfp281 on 2C-like transition, all results are analyzed at 12 h after synDux induction, when most mESCs are transiting into 2C-like state (Fu et al., 2019). (E) The relative percentage of 2C-like cells in mESCs upon indicated manipulation. (B–E) The siTet1-1/2 represents two independent siRNA targeting Tet1, siGFP is the negative control. Dox represents doxycycline. The X in sgX refers to the gene that sgRNA targets to; sgSv40 is negative control. Shown are mean ± s.d, n = 3. p Values were calculated by the parametric one-way analysis of variance (ANOVA) test, ns = no significance, ** <0.01, *** <0.001, **** <0.0001.