FIGURE 4.

Zfp281 leads to an impaired 2C-like-transition ability in primed-state mESCs. (A) The percentage of 2C-like cell population in ground-naïve (2iL), metastable-naïve (SL), and primed states (FA) mESCs upon indicated perturbation. Representative FACS results are shown. (B) The fold change of the 2C-like cell population in three pluripotent states Zfp281-perturbed mESCs normalized to control mESCs. Redline indicates fold change of 1. (A–B) Shown are mean ± s.d, n = 3. p Values were calculated by unpaired t-test, two-tailed, two-sample unequal variance, * <0.05, ** <0.01, *** <0.0001. (C) A box plot demonstrating the log₂ (fold change) of 2C-upregulated genes expression in metastable-naïve and primed states mESCs upon Zfp281 perturbation. The original RNA-seq was downloaded from GSE81045. p Values were calculated by the Wilcoxon rank-sum test, * <0.05. The dots represent outliers. (D) A model showing Zfp281 and Tet1 contributes to the impaired 2C-like-transition ability by repressing the expression of 2C-upregulated genes. (A–C) Dox represents doxycycline. The X in sgX refers to the gene that sgRNA targets to; sgSv40 is negative control.