FIGURE 3.

Antigenicity and immunogenicity of PfCyRPA produced in insect and mammalian cells. (A) Binding of anti-PfCyRPA mAbs recognizing different epitopes on purified PfCyRPA protein by ELISA. (B) In vitro parasite growth-inhibitory activity of polyclonal IgG antibodies generated in rabbits upon immunization with High Five cells derived PfCyRPA. Data are shown as mean ± standard deviation of triplicate wells of a single assay, and are representative of two independent runs. For each IgG preparation a four-parameter sigmoidal dose–response curve was fitted to the relationship between log₁₀ (antibody concentration) and % inhibition and then used to interpolate IC₅₀ values (antibody concentrations giving 50% growth inhibition).