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. 2022 May 20;10:879078. doi: 10.3389/fbioe.2022.879078

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Copyright © 2022 Fernandes, Sousa, Castro, Schäfer, Hauser, Schulze, Amacker, Tamborrini, Pluschke, Alves, Fleury and Roldão.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Design of experiments (DoE) for optimizing PfCyRPA production in High Five cells. Response surface models for PfCyRPA protein titer (mg/L) from screening experiments (A) and optimization experiments (B). (C) Cell growth and viability kinetics in optimal conditions identified by DoE. Improvement of PfCyRPA protein titer (mg/L) in optimal conditions identified by DoE vs. standard condition (i.e., cultures at 27°C and no supplementation). DoE images were obtained using MODDE® 12.1 Pro software (Sartorius). Data in bar graphs are expressed as mean ± standard deviation (relative to three technical replicates, n = 3). Abbreviations: TOI, time of infection; Lip, lipids; Poly, polyamines; α-keta, α-ketoglutarate; AOx, antioxidants.