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. 2022 May 20;10:879078. doi: 10.3389/fbioe.2022.879078

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Copyright © 2022 Fernandes, Sousa, Castro, Schäfer, Hauser, Schulze, Amacker, Tamborrini, Pluschke, Alves, Fleury and Roldão.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impact of affinity tags on PfCyRPA production in High Five cells. (A) Cell growth and viability kinetics obtained in STB cultures. (B) PfCyRPA final yield (mg/L) after purification. (C) SDS-PAGE of purified PfCyRPA, under reduced (R) and non-reduced (NR) conditions; Ladder (L) is SeeBlue™ Plus2 Pre-stained Protein Standard. (D) Dynamic light scattering profile of purified PfCyRPA. (E) Peptide identification of purified PfCyRPA, digested with LysC, trypsin, and AspN, by LC-MS using X500B QTOF (ABSciex). Color code: PfCyRPA-His6 (blue); PfCyRPA-His4 (green); PfCyRPA-Ctag (yellow). Data in bar graphs are expressed as mean ± standard deviation (relative to three technical replicates, n = 3). Abbreviations: AOx, antioxidant.