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. 2022 May 20;9:857933. doi: 10.3389/fcvm.2022.857933

FIGURE 1.

FIGURE 1

ApoE deficiency resulted in atherosclerosis with a significant increase in serum ferritin and aortic iron and ferritin in mice. The contents of ferritin (ng/mL) (A), iron (μmol/L) (B) and Tf saturation (%) (C) in the serum, and Oil Red O staining (D), DAB-enhanced Perls’ iron staining (E), immunohistochemical analysis of FTL protein expression (F), content of iron (mg/g wet weight) (G), expression of FTL mRNA (H), FTH mRNA (I), FTL protein (J,L) and FTH protein (K,L) in the aortic tissues were determined or performed by the procedures as described in section “Materials and Methods” in WT (n = 10; WB: n = 4), ApoE– /– (n = 10; WB: n = 4) and ApoE– /– + DFO (n = 10; WB: n = 4) mice, all aged 28 week-old. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in Hanks’ balanced salt solution (HBSS) for 14 days. Scale bar in panels (D–F) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.