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. 2022 May 20;9:857933. doi: 10.3389/fcvm.2022.857933

FIGURE 2.

FIGURE 2

Increased expression of transferrin receptor 1 in the aortic tissues of ApoE deficient mice. The expression of TfR1 and Fpn1 proteins [TfR1, (A,B); Fpn1, (A,C)] and mRNAs [TfR1, (D); Fpn1, (E)] in the aortic tissues were, respectively, determined by Western blot and RT-PCR analysis as described in section “Materials and Methods” in WT (n = 8; WB: n = 4), ApoE– /– (n = 8; WB: n = 4) and ApoE– /– + DFO (n = 8; WB: n = 4) mice, all aged 28 week-olds. Immunohistochemical examination of TfR1 protein expression in the aortic tissues (F) was also conducted in the three groups of mice. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in HBSS for 14 days. Scale bar in panel (F) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.