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. 2022 May 20;9:857933. doi: 10.3389/fcvm.2022.857933

FIGURE 3.

FIGURE 3

ApoE deficiency up-regulated IRP1 and IRP2 expression but not hepcidin in the aortic tissues. The expression of IRP1 and IRP2 proteins [IRP1, (A,B); IRP2, (A,C)], mRNAs [IRP1, (D); IRP2, (E)] and hepcidin mRNA (F) in the aortic tissues were, respectively, determined by Western blot and RT-PCR analysis as described in section “Materials and Methods” in WT (n = 8; WB: n = 3), ApoE– /– (n = 8; WB: n = 3) and ApoE– /– + DFO (n = 8; WB: n = 3) mice, all aged 28 week-old. Immunofluorescence analysis of IRPs protein expression in the aortic tissue (G,H) was also conducted in the three groups of mice. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in HBSS for 14 days. Scale bar in panel (H) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.