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. 2022 May 20;9:857933. doi: 10.3389/fcvm.2022.857933

FIGURE 4.

FIGURE 4

ApoE deficiency up-regulated HO1 expression in the aortic tissues. The expression of HO1 protein and mRNA in the aortic tissues were, respectively, determined by Western blot (A,B), RT-PCR (C) or/and immunofluorescence (D) analysis as described in section “Materials and Methods” in WT (n = 8; WB: n = 4), ApoE– /– (n = 8; WB: n = 4) and ApoE– /– + DFO (n = 8; WB: n = 4) mice, all aged 28 week-old. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in HBSS for 14 days. Scale bar in panel (D) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.