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. 2022 May 20;9:857933. doi: 10.3389/fcvm.2022.857933

FIGURE 5.

FIGURE 5

ApoE deficiency up-regulated the expression of ICAM1, VCAM1, LOX-1, CD36, Gpx4, TNFα, IL-1β, and IL-6 in the aortic tissues of mice. The expression of ICAM1, VCAM1 and Gpx4 proteins in the aortic tissues were measured by Western blot [ICAM1, (A,C); VCAM1, (A,D); Gpx4, (A,E)] or/and immunohistochemistry [ICAM1, (B)] analysis, and ICAM1 (F), VCAM1 (G), Gpx4 (H), LOX-1 (I), CD36 (J), and inflammatory cytokines TNFα, IL-1β, IL-6 and IL-10 (K) mRNAs by RT-PCR analysis as described in section “Materials and Methods” in WT (n = 6; WB: n = 4), ApoE– /– (n = 6; WB: n = 4) and ApoE– /– + DFO (n = 6; WB: n = 4) mice, all aged 28 week-old. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in HBSS for 14 days. Scale bar in panel (B) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.