FIGURE 6.
Increased production of reactive oxygen species, NF-κ B phosphorylation and reduced SOD and GSH-PX contents in ApoE deficient mice. The contents of 8-isoprostane (pg/mL) (A), MDA (nmol/mL) (B), H2O2 (nmol/mL) (C), ROS (Fold of the Control) (D) and ox-LDL (μg/mL) (E), SOD (U/mL) (F) and GSH-PX (G) in the blood, and NF-κB phosphorylation [(H,I) NF-kB family contains five members: p50 (NF-kB1), p52 (NF-kB2), p65 (RelA), c-Rel, and RelB, and panel (J)] in the aortic tissues were determined by the procedures as described in section “Materials and Methods” in WT (n = 8), ApoE– /– (n = 8) and ApoE– /– + DFO (n = 8) mice, all aged 28 week-old. The mice in ApoE– /– + DFO group received daily i.p. injections of 100 mg/kg body weight (b.w.) of DFO in HBSS for 14 days. Scale bar in panel (J) = 50 μm. Data are presented as the mean ± SEM. *p < 0.05.