Table 1.
Comparison of determination methods of molecular weight.
| Method | Characteristic | Application | References | |
|---|---|---|---|---|
| Traditional techniques | Osmotic pressure method, Viscosimetry, Vapor pressure osmometry, Terminal method, Sugar Electrophoresis, Ultrafiltration | Complicated to operate, inaccurate | Tea polysaccharide (TPS) Mw 2.287 × 105-2.762 × 105 g/mol | (69) |
| GPC | HPSEC/HPGPC-RID | Fast, convenient for detection, high resolution and good reproducibility, homogeneity and molecular weight can be detected simultaneously | Bletilla striata polysaccharide (pFSP) Mw 9.1 × 104 Da | (4) |
| HPSEC/HPGPC-ELSD | Lepidium meyenii polysaccharide (MP1) Mw 4.67 × 105 Da | (70) | ||
| Combined techniques | HPSEC/HPGPC-MALLS | Less affected by sample structure or relative molecular weight, not necessary to use reference materials for calibration, high accuracy, can provide samples conformational information in solution | Ligusticum chuanxiong hort polysaccharide (LCP70-2A) Mw 6.46 × 104 | (71) |