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. 2022 Jun 3;414(21):6309–6326. doi: 10.1007/s00216-022-04131-2

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Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — 8% SDS-PAGE displaying R5₂-mCh-H10-BST_LF (99.3kDA): A Immobilisation in Tris buffer (insert shows Eppendorf containing Si-R5₂-mCh-H10-BST_LF): (1) cell pellet, (2) cell lysate, (3) supernatant after silica adsorption, (4) silica protein desorption, post-protein adsorption, (5) supernatant after nickel-resin purification, (6) elution from nickel-resin, (7) supernatant after silica adsorption of nickel-resin elution, (8) silica post-nickel-resin elution adsorption. R5₂-mCh-FL-BST_LF (98.6kDA): (9) cell pellet, (10) cell lysate, (11) supernatant after silica adsorption, (12) silica protein desorption, post-protein adsorption. B Eight percent SDS-PAGE highlighting PBS-based silica adsorption of R5₂-mCh-H10-BST_LF (99.3kDA) (insert shows Eppendorf containing Si-R5₂-mCh-H10-BST_LF): (13) cell pellet, (14) cell lysate, (15) supernatant after silica adsorption, (16) silica protein desorption, post-protein adsorption