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. 2022 Jun 3;414(21):6309–6326. doi: 10.1007/s00216-022-04131-2

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Fig. 6 — qPCR performed on output from BST_LF DNA amplification (15 min) targeting 100 copies of P. knowlesi using only the F3 and B3 primers from the P.KNO-LAU primer set. Activity assay, A (i) commercial BST_LF (8 U); (ii) Si-R5₂-mCh-FL-BST_LF in ThermoPol® buffer with added Mg²⁺; (iii) 0.2 × isothermal amplification buffer with added Mn²⁺ and Mg²⁺; (iv) Si-R5₂-mCh-FL-BST_LF immobilised in PBS and used in ThermoPol® buffer with added Mg²⁺. B Comparing the recombinant BST_LF on silica in ThermoPol® buffer, supplemented with different concentrations of Mn²⁺ and Mg²⁺. 1U of BST_LF is defined as the amount of enzyme required to incorporate 10 nmol of dNTP in 30 min at 65 °C.