. 2022 Jun 3;414(21):6309–6326. doi: 10.1007/s00216-022-04131-2

Table 1.

Ninety-minute limit of detection comparison between LAMP run with commercial enzyme or recombinant enzyme depending on Plasmodium target and primer set (Lau et al. or Han et al.)

Malaria primer set	Limit of detection per reaction
Malaria primer set	Commercial BST_LF	Si-R5₂-mCh-FL-BST_LF
18S rRNA (Lau et al.)
P. falciparum	10¹ copies^b	No activity
P. malariae	10⁴ copies	No activity
P. vivax	10¹ copies	10⁵ copies
P. ovale	10⁵ copies^b	No activity
P. knowlesi	10² copies	10³ copies
18S rRNA (Han et al.)
P. falciparum	10³ copies^a	10² copies^a
P. vivax	10⁴ copies^b	10³ copies
P. ovale	10⁵ copies^b	10¹ copies
Mitochondrial DNA
P. falciparum	10² copies^a	10¹ copies^a

^aLoD test used genomic P. falciparum Dd2 DNA.

^bPrimer set had a high tendency for false positives.