TABLE 1.
Cd2+ (μM) | Cd2+ uptake (nmol g of dry cell−1 min−1) by:
|
||
---|---|---|---|
L. plantarum ATCC 14917
|
E. coli XL1-blue | ||
Mn2+ starved | Mn2+ sufficient | ||
0.02 | 73 ± 6 | 0.5 ± 0.1 | 0.3 ± 0.1 |
0.2 | 802 ± 42 | 6.1 ± 0.3 | 1.8 ± 0.2 |
2 | 2,821 ± 112 | 52 ± 3 | 5.8 ± 0.52 |
20 | 3,563 ± 308 | 258 ± 16 | 23 ± 1 |
100 | 3,214 ± 382 | ND | 41 ± 10 |
200 | 3,082 ± 203 | 520 ± 42 | 45 ± 7 |
Cells were prepared and preincubated for 10 min at 37°C in low-Mn2+ APT medium containing different amounts of Cd2+ as described in the text. Initial uptake values were determined with 0.2-ml samples filtered 15 s after radioactive Cd2+ was added. Values are means ± ranges from replicate experiments. ND, not determined.