MeV fusion inhibitor peptides: design and structural features. IC50 and IC90 values for fusion inhibition by [FIP–PEG4]2–chol, [HRC–PEG4]2–chol, and [FIP–HRC–PEG4]2–chol. HEK 293T cells transiently transfected with nectin-4 and the omega reporter subunit of β-gal (“target cells”) were incubated with cells coexpressing viral glycoproteins (H and F) and the alpha reporter subunit of β-gal (“effector cells”) in the presence or absence of inhibitory peptides. In the absence of peptides, fusion between the target and effector cells permits reconstitution of β-galactosidase activity, quantified using the luminescence-based kit, Galacto-Star β-galactosidase reporter gene (ThermoFisher). The data presented are the means of three independent experiments (±SE).