Figure 3.

Sostdc1 expression levels were significantly increased during the anagen phase. (A) LECs were incubated with 400 ng/mL VEGF-C for 24 h, followed by qRT-PCR to assess Sostdc1 expression. (B) Total RNA was isolated from the back skin of female C57BL/6 mice and Sostdc1 expression was determined by qRT-PCR. (C) DPCs were treated with the indicated concentrations of Sostdc1 for 72 h and Lef-1 mRNA expression was determined by qRT-PCR. (D) DPCs were incubated with 50% LEC-CM-Sostdc1 shRNA or LEC-CM-scrambled shRNA for 48 h. Total RNA was isolated and mRNA expression levels of WNT7b, BMP2 and BMP4 were measured by qRT-PCR. Results are expressed as mean ± SEM. Data were analyzed using a two-tailed unpaired t-test. * p < 0.05, ** p < 0.01 compared to the control group.