Table 1.

Parameters evaluated in platelet concentrates.

Parameters	Day 1	Day 2	Day 3	Day 4	Day 5	Day 6	Day 7
* Quality control
Swirling	Positive	Positive	Positive	Positive	Positive	Negative	Negative
PC volume	3 mL	3 mL	3 mL	3 mL	3 mL	3 mL	3 mL
Leukocyte count (108/70 mL)	0.51 ± 0.02	0.48 ± 0.02	0.42 ± 0.03	0.34 ± 0.03	0.25 ± 0.04 *	0.18 ± 0.06 *	0.12 ± 0.06 *
Platelet count (1010)	7.3 ± 0.10	6.9 ± 0.11	6.7 ± 0.15	6.4 ± 0.18	6.1 ± 0.21	5.8 ± 0.24	5.5 ± 0.30
pH	7.2 ± 0.02	7.2 ± 0.03	7.2 ± 0.01	7.3 ± 0.01	7.3 ± 0.02	7.3 ± 0.01	7.4 ± 0.05
miRNAs expression **
miR-127	0.30 ± 0.12	0.41 ± 0.16	0.43 ± 0.19	0.32 ± 0.17	0.24 ± 0.23	0.29 ± 0.21	0.35 ± 0.25
miR-320a	0.19 ± 0.11	0.27 ± 0.13	0.37 ± 0.13 *	0.53 ± 0.18 *	0.54 ± 0.19 *	0.56 ± 0.22 *	0.62 ± 0.22 *
Membrane proteins’ expression +
P2Y12	39.57 ± 2.14	39.48 ± 1.73	40.56 ± 1.97	41.64 ± 1.56	41.19 ± 1.28	42.87 ± 1.80	42.69 ± 1.32
CD62P	11.45 ± 3.12	13.76 ± 4.61	18.96 ± 5.22	21.70 ± 6.22 *	33.76 ± 6.25 *	38.26 ± 7.11 *	40.20 ± 7.17 *
CD41	94.32 ± 4.20	89.81 ± 2.81	87.54 ± 2.52	85.73 ± 1.98	80.11 ± 2.53 *	78.81 ± 3.22 *	75.54 ± 2.60 *
CD61	87.62 ± 3.43	83.51 ± 3.72	80.31 ± 3.53	78.13 ± 2.46	77.14 ± 3.25 *	75.21 ± 3.27 *	73.11 ± 4.10 *

Values are expressed as means ± standard deviations. * Gravimetric measurement. ** Values referring to the relative quantification of miRNAs mir127 mir320a, for mir-191. + Percentage of platelets positive for CD62P and for GPIIb and -IIIa. For the P2Y12 receptor, the mean fluorescence intensity (MFI) was evaluated.