FIG. 3.

Deduced amino acid sequence of the 170-kDa Cry1Ac-binding protein. The 3,198-bp cDNA clone encodes a 1,010-amino-acid polypeptide with a predicted cleavage site at Ser-20, which follows a signal sequence (dot underlining). The N terminus obtained from peptide sequencing (solid underlining), the canonical metal-binding site (box), the GPI anchor addition site (boldface underlining), and the cleaved C-terminal hydrophobic sequence (dash underlining) are shown. Predicted sites for N glycosylation (double underlining) and O glycosylation (dots) are also indicated. The polythreonine-rich stretch extends from amino acid 939 to amino acid 984. The GenBank accession number for this sequence is AF173552.