FIG. 5.
In vitro transcription-translation and immunoblot analysis of the 130- and 170-kDa proteins. In vitro-produced mRNA was translated in a rabbit reticulocyte system; 35S-labeled proteins were separated on a 10% polyacrylamide SDS-PAGE gel and visualized by autoradiography; and BBMV and 1 μg of pool I protein were separated on an 8% polyacrylamide SDS-PAGE gel and transferred to an Immobilon-P membrane. Lane 1, in vitro transcripton and translation of the cDNA clone; lanes 2 through 5, immunoblot analysis of pool I proteins performed with 170-kDa protein antiserum (lanes 2 and 3) and 120-kDa protein antiserum (lanes 4 and 5) (lanes 2 and 4, BBMV; lanes 3 and 5, pool I protein); lanes 6 and 7, SBA lectin blot analysis of PNGase-treated (lane 7) and control (lane 6) pool I protein.