Table 1. Immune cell subsets and phenotypes commonly used in research and identified by flow cytometry.

Immune cell subtypes and their phenotypes are summarized based on the currently available literature. Differences between mouse and human cells exist as determined by expression of surface markers specific to the given subset of immune cells.

Model	Cell type	Subtype	Phenotype	References
Mouse	T cells	Cytotoxic T cells	CD3+, CD8+	[31, 92]
		Helper T cells	CD3+, CD4+	[31, 92]
		Regulatory T cells	CD3+, CD4+, CD25+, FoxP3+	[92]
	B cells		CD19+, B220+	[31, 32, 92]
	NK cells		CD3+, NKp46+	[31, 32, 92]
	Macrophages		CD11b+, F4/80+, CD68+	[92]
	DCs	mDCs	CD11b+, CD11c+, CD80+, CD86+	[92]
		pDCs	CD11b−, CD11c+, B220+, Siglec- H+	[33, 92]
	Monocytes		CD11b+, CD68+, Ly6c+	[32, 33, 92]
	Neutrophils		CD11b+, Gr-1+, Ly6b+, F4/80−	[92]
Human	T cells	Cytotoxic T cells	CD3+, CD8+	[25, 31]
		Helper T cells	CD3+, CD4+	[25, 31]
		Regulatory T cells	CD3+, CD4+, CCR4+, CD25+, CD127low	[25]
		Naïve T cells	CCR7+, CD45RA+	[25, 93]
		Effector T cells	CCR7−, CD45RA+	[25]
		Effector Memory T cells	CCR7−, CD45RA−	[25, 93]
		Central Memory T cells	CCR7+, CD45RA−	[25, 93]
	B cells	Naïve B cells	CD3−, CD19+, CD27−	[25, 33]
		Memory B cells	CD3−, CD19+, CD27+	[25, 33]
	NK cells		CD3−, CD19−, CD14−, CD20−, CD56+, CD16+/−	[25, 31, 32]
	NK T cells		CD3+, CD56+	[25]
	DCs	mDCs	CD3−, CD19−, CD14−, CD20−, HLA-DR+, CD11c+	[25, 31–33]
		pDCs	CD3−, CD19−, CD14−, CD20−, HLA-DR+, CD123+	[25, 31, 33]
	Monocytes	Classical	CD3−, CD19−, CD14+, CD16−	[25]
		Non-classical	CD3−, CD19−, CD14+, CD16+	[25]
	Neutrophils		CD15+, CD16+, CD14low	[25]