Figure 6.

GhTCP4-like interact with GhNPR1. (A) Yeast two-hybrid (Y2H) assay shows GhTCP4-like interaction with GhNPR1. All recombination yeast cells were grown on SD/-Trp/-Leu medium. After gradient dilution and sprayed 10 mM 3-AT and 20 mg/ml X-α-Gal on SD/-Trp/-Leu /-His/-Ade medium. Yeast transformed with paired plasmid AD-largeT and BD-p53 were used as a positive control, AD-largeT and BD-laminC were used as a negative control. Photos were taken after 3 d at 28°C. (B) LCI assays to detect the association between GhTCP4-like and GhNPR1. Agrobacterium strains containing the indicated vectors were co-expressed in leaf cells of N. benthamiana. The luminescence signal was detected by applying 1 mM luciferin after 48 h of agroinfiltration. The positive control was constructed according to Chen et al. (2008). The colored scale indicates the intensity of luciferin activity. All experiments were repeated more than three times with similar results.