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. Author manuscript; available in PMC: 2022 Jun 4.
Published in final edited form as: Nature. 2021 Sep 29;598(7881):515–520. doi: 10.1038/s41586-021-03951-z

Extended Data Figure 9. Cryo-EM single particle reconstruction of GsuCas4/Cas1-Cas2 programmed with a half-integration mimic.

Extended Data Figure 9.

a. Workflow of cryo-EM data processing. b. Overall cryo-EM density showing resolution distribution, viewing direction distribution plot, and FSC curves of three different snapshots. Left: half-integration, Cas4 disappeared; Middle: full-integration; Right: half-integration, Cas4 still blocking PAM-side.