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. 2022 May 20;13:907901. doi: 10.3389/fmicb.2022.907901

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Copyright © 2022 Chen, Yao, Wang, Fen, Chen, Xu, Yu, Li and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Culture of organoids from bile ducts and small intestines of mice. After gravity settling and multi-step centrifugation, cholangiocytes (derived from bile ducts), bile ducts, and crypts from the small intestine formed organoids after ~14 days in culture. Cholangiocytes (day 0) from (A) bile ducts formed cholangiocyte organoids (cho-orgs) (day 3, passage 2) (bar, 100 μm), and those from (B) intrahepatic bile ducts (day 0) formed cho-orgs (day 3) (bar, 100 μm). (C) Crypts from the small intestine (day 0) (bar, 200 μm) formed small intestinal organoids (in-orgs) (day 13) (bar, 100 μm).