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Characteristics of small intestinal organoids (in-orgs). (A) Immunofluorescence staining for the enterocyte cell marker CK20 (red) (bar, 100 μm). (B) Immunofluorescence staining for the complete tight junction protein CLAUDIN-1 (red) (bar, 100 μm). (C) Fluorescent dye FITC-dextran-4KD (FD4) absorbing results, observed with a laser confocal Microscope. The in-orgs are on a green background (bar, 100 μm).
