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. 2022 May 25;48(1):127. doi: 10.3892/or.2022.8338

Figure 2.

Figure 2.

The lentiviral vector schematic maps and immunophenotype of MSLN-SmartDC and RPS3-MSLN-SmartDC. (A) Construction of MSLN-SmartDC lentiviral vector. (B) Expression of MSLN protein in Lenti-X™ 293T transfected with IRFP-SmartDC and MSLN-SmartDC lentiviral vectors. (C) Representative images of monocytes at day 0 and DCs at day 7 of the experiment. Production of (D) GM-CSF, (E) IL-4 and (F) IL-12p70 by monocytes and different DCs. The geometric mean fluorescence intensity of surface markers of DCs in different treatment conditions including (G) CD14, (H) CD40, (I) HLA-DR, (J) CD80, (K) CD83 and (L) CD86 in monocytes and DCs. Original magnification, ×100 and scale bar=50 µm. The results were collected from four independent experiments. ND, not detected. #P<0.05, ##P<0.01 and ###P<0.001 vs. monocytes. **P<0.01 and ***P<0.001. MSLN, mesothelin; MSLN-SmartDC, MSLN dendritic cells; RPS3, ribosomal protein subunit 3; GM-CSF, granulocyte-macrophage colony stimulating factor; DCs, dendritic cells; HLA, human leukocyte antigen.