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. 2022 May 17;48(1):122. doi: 10.3892/or.2022.8333

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — CSF2 is the main difference gene between the adherent and the suspended SCLC phenotypes. H69A and H69S were detected with RNA sequencing. (A) Heat map representation of 404 differentially expressed genes. The significantly enriched GO annotations in (B) Biological Process, (C) Cellular Components and (D) Molecular Function analysis of 404 differentially expressed genes. (E) KEGG pathway analysis of 404 different genes. (F) H69A and H69S cells were collected and CSF2 mRNA levels were evaluated with reverse-transcription quantitative PCR by using the 2-^ΔΔCq method. (G) CSF2 was tested by using western blotting. GAPDH was used as the internal standard (*P<0.05, vs. the adherent group). (H) Quantitative analysis of (G): H69A was used as the control (100%). Data are presented as the mean ± standard deviation of three independent experiments (*P<0.05). SCLC, small cell lung cancer; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.