Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 3;19:39. doi: 10.1186/s12987-022-00341-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 1 — Identification and expression analysis of causative gene in H-Tx rats. A The log2 ratio value is plotted on the x-axis. The y-axis represents the genomic position of probes. Genomic profiles indicate a 14-kbp focal loss in 16p16. Colored vertical lines indicate the number of samples with copy number loss and loss segments (brown square) with respect to Ptpn20. B Real-time PCR of copy number in the Ptpn20 exon of H-Tx (−) and H-Tx (+) rats at E18. exon 6: H-Tx (−) = 2.11 ± 0.78, H-Tx (+) = 0.88 ± 0.47; exon 7: H-Tx (−) = 1.84 ± 0.37, H-Tx (+) = 1.15 ± 0.25 (mean ± standard deviation, **P < 0.01, ***P < 0.001; H-Tx (−) rats, n = 12; H-Tx (+) rats, n = 6). C Real-time PCR of RNAs from P1 rats in SD, Wistar, H-Tx (−) and H-Tx (+) groups to determine expression levels of Ptpn20 in the brain. Data are normalized to β-actin and fold change is expressed relative to levels in the SD rat. SD = 1.00 ± 0.14; Wistar = 0.98 ± 0.15; H-Tx (−) = 1.20 ± 0.08; H-Tx (+) = 0.55 ± 0.19 (mean ± standard deviation, **P < 0.01, ***P < 0.001; n = 5 rats per group). D Immunoblot analysis of Ptpn20 protein from the CP of P1 H-Tx (−) and H-Tx (+) rats. The 50-kDa bands correspond to Ptpn20 and nonspecific (n.s.) bands, respectively. Ptpn20 protein shows low or approximately abolished expression in H-Tx (+) rats. Actin was used as a loading control (n = 5 rats per group). E Immunofluorescence images of the CP in P1 SD, H-Tx (−) and H-Tx (+) rats are immunolabeled with anti-Ptpn20 (green) antibody and with DAPI (blue). Ptpn20 appears enriched in the CP epithelium of SD and H-Tx (−) rats, with low expression in H-Tx (+) rats, Scale bar = 20 μm