FIG. 1.

RT-PCR on T. ferrooxidans total RNA. (A) Localizations of the oligonucleotides used for RT-PCR experiments; (B) RT-PCR experiments with oligonucleotides a and b on no template (lane 0), T. ferrooxidans genomic DNA (lane D), total RNA from T. ferrooxidans cells (lane R), total RNA from T. ferrooxidans but without reverse transcriptase (lane −); (C) RT-PCR experiments with oligonucleotides c and i, d and i, and f and i on RNA extracted from sulfur-grown cells (lanes R and −); (D) RT-PCR experiments with oligonucleotides e and i, f and i, and g and j on RNA extracted from ferrous-iron-grown cells (lanes R and −). Lanes M, 1-kb molecular weight ladder from Boehringer Mannheim.