Fig. 7.

S100A8/A9-RAGE system prompts growth effects in TNBC cells. A Cell proliferation in MDA-MB231 cells overexpressing RAGE upon stimulation for 72 hours with 100 ng/ml rhS100A8/A9 used alone or in combination with 1 μM RAGE antagonist FPS-ZM1 or 1 μM FAK inhibitor VS-4718. B Cell proliferation in BT-549 cells overexpressing RAGE upon stimulation for 72 hours with 100 ng/ml rhS100A8/A9 used alone or in combination with RAGE antagonist 1 μM FPS-ZM1 or 1 μM FAK inhibitor VS-4718. C-D Colony formation assay in BT-549 cells overexpressing RAGE and treated for 10 days with 100 ng/ml rhS100A8/A9 alone or in combination with 1 μM RAGE antagonist FPS-ZM1m or 1 μM FAK inhibitor VS-4718. E Cell proliferation in BT-549 cells overexpressing RAGE and transfected with siRNA control (20 nM) and siRNA targeting CTGF (20 nM) and stimulated for 72 hours with 100 ng/ml rhS100A8/A9. F-G Colony formation assay in BT-549 cells overexpressing RAGE transfected with siRNA control (20 nM) and siRNA targeting CTGF (20 nM) and stimulated for 10 days with 100 ng/ml rhS100A8/A9. H CTGF knockdown efficiency is shown. Error bars represent mean ± SD. * p-value < 0.05 indicates. Results shown are representative of three independent experiments performed in triplicate