Skip to main content
. 2022 Feb 18;10(5):597–611. doi: 10.1158/2326-6066.CIR-21-0129

Figure 3.

Figure 3. Bhlhe40 is required for ICT-induced remodeling of the intratumoral immune cells. A, UMAP plot from scRNAseq of intratumoral CD45+ cells harvested on day 9 posttumor transplant from 1956 tumor–bearing Bhlhe40+/+ or Bhlhe40–/– mice treated with control mAb, anti–CTLA-4, or anti–PD-1. Cluster cell types identified via known cellular subset marker expression and comparison with Immgen database. B, UMAP plots from scRNAseq of CD45+ intratumoral cells. C, Heatmap displaying normalized expression of select genes in each cell cluster by group. D, Violin plot showing Bhlhe40 expression by cluster.

Bhlhe40 is required for ICT-induced remodeling of intratumoral immune cells. A, UMAP plot from scRNAseq of intratumoral CD45+ cells harvested on day 9 posttumor transplant from 1956 tumor–bearing Bhlhe40+/+ or Bhlhe40–/– mice treated with control mAb, anti–CTLA-4, or anti–PD-1. Cluster cell types identified via known cellular subset marker expression and comparison with Immgen database. B, UMAP plots from scRNAseq of CD45+ intratumoral cells. C, Heatmap displaying normalized expression of select genes in each cell cluster by group. D, Violin plot showing Bhlhe40 expression by cluster.