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. 2022 Feb 18;10(5):597–611. doi: 10.1158/2326-6066.CIR-21-0129

Figure 4.

Figure 4. Bhlhe40 regulates CD4+ and CD8+ T-cell function. Intratumoral CD45+ cells were harvested on day 9 posttumor transplant from 1956 tumor–bearing Bhlhe40+/+ or Bhlhe40–/– mice treated with control mAb, anti–CTLA-4, or anti–PD-1, and analyzed by scRNAseq. A, UMAP displaying reclustering of T cell–containing clusters (middle plot) and Cd4 and Cd8 expression (bottom plot) of all experimental conditions computationally combined. B, Heatmap displaying normalized expression of select genes in each T-cell cluster. C, Violin plot showing Bhlhe40 expression in Bhlhe40+/+ mice by cluster and treatment. D, Percentage of cells in individual T-cell clusters by condition and treatment represented as percent of CD45+ cells.

Bhlhe40 regulates CD4+ and CD8+ T-cell function. Intratumoral CD45+ cells were harvested on day 9 posttumor transplant from 1956 tumor–bearing Bhlhe40+/+ or Bhlhe40–/– mice treated with control mAb, anti–CTLA-4, or anti–PD-1, and analyzed by scRNAseq. A, UMAP displaying reclustering of T cell–containing clusters (middle plot) and Cd4 and Cd8 expression (bottom plot) of all experimental conditions computationally combined. B, Heatmap displaying normalized expression of select genes in each T-cell cluster. C, Violin plot showing Bhlhe40 expression in Bhlhe40+/+ mice by cluster and treatment. D, Percentage of cells in individual T-cell clusters by condition and treatment represented as percent of CD45+ cells.