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Engineered B. subtilis strain for de novo synthesis of 2ʹ-FL using sucrose and lactose as carbon sources. Green arrows and genes indicate a heterogenous 2′-FL de novo biosynthetic pathway. Serial numbers represent the corresponding metabolic reactions. Abbreviations: F-1,6-P, fructose-1, 6-diphosphate; F6P, fructose-6-phosphate; G6P, glucose-6-phosphate; GDP-F, GDP-L-fucose; GDP-M, GDP-mannose; M1P, mannose-1-phosphate; PEP, phosphoenolpyruvate; sucrose-6P, sucrose-6-phosphate
