Figure 3.

VE607 stabilizes SARS-CoV-2 S in the “up” conformation

(A) VE607 does not compete for sACE2 interaction as measured by flow cytometry. The values represent the median fluorescence intensities (MFI) normalized to binding signals obtained with the conformationally independent CV3-25 Ab. Five experiments are represented as mean ± SEM and statistical significance was tested using unpaired t-test.

(B) SARS-CoV-2 Spike stability was measured by radioactive labeling of 293T Spike expressing cells followed by immunoprecipitation of cell lysates and supernatants. At least four experiments are represented as mean ± SEM and statistical significance was tested using unpaired t-test, ∗p < 0.05.

(C–E) Single molecule FRET analysis of SARS-CoV-2 S unliganded (C), in presence of sACE2 (D) or VE607 (E).