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[Preprint]. 2022 May 27:rs.3.rs-1690354. [Version 1] doi: 10.21203/rs.3.rs-1690354/v1

Figure 6. NEIL2 modulates SARS-CoV-2 protein synthesis.

Figure 6

a, b) Human BEAS-2B cells, control or stably expressing NEIL2-FLAG protein (NEIL2) were transfected with CoV2-5’-UTR-eGFP construct (a) or a plasmid devoid of CoV-2-5’-UTR (UTR-Less-eGFP) (b) and GFP protein expression was analyzed in live cells after 16 h. Representative images from at least 10 randomly selected frames (left panels). Right panels represents GFP Fluorescence per μM2 from at least 10 randomly selected frames. c, d) HEK293 cells were transfected with Control or -NEIL2 specific siRNA for 48 h, then transfected with CoV2-5’-UTR-eGFP construct (c) or UTR-Less-eGFP plasmid (d), and GFP fluorescence per μM2 was measured in live cells from at least 10 randomly selected frames, p-values calculated from at least three biological replicates.