Table 1.
Primary antibodies used for immunohistochemistry (IHC) and Western blot analysis (WB)
| Antibody name | Use | Concentration | Company | Catalogue No. |
|---|---|---|---|---|
| CK19 | IHC | 1:400 | Abcam | Ab133496 |
| CK7 | IHC | 1:400 | Abcam | Ab181598 |
| HNF4α | IHC | 1:100 | Abcam | Ab181604 |
| HNF1B | IHC | 1:50 | Proteintech | 12533-1-AP |
| Periostin | IHC | 1:100 | Abcam | ab215199 |
| Osteopontin | IHC | 1;100 | Abcam | ab283656 |
| Hydroxyproline | IHC | 1:100 | Cell Signaling Technology | 73812 |
| SOX9 | IHC | 1:500 | Cell Signaling Technology | 82630 |
| Vimentin | IHC | 1:1000 | Abcam | ab92547 |
| Phospho-TAZSer89 | IHC | 1:100 | Cell Signaling Technology | 59971 |
| V5-tag | IHC | 1:100 | Abcam | ab95038 |
| Yap | IHC; WB | 1:100; 1:1000 | Cell Signaling Technology | 14074 |
| α-SMA | IHC | 1:400 | Abcam | ab5994 |
| TAZ/WWTR1 | IHC | 1:100 | Sigma-Aldrich | AMab90730 |
| TAZ | IHC | 1:200 | Cell Signaling Technology | 72804 |
| Yap/Taz | WB | 1:1000 | Cell Signaling Technology | 8418 |
| Notch1 | IHC | 1:100 | Cell Signaling Technology | 3608 |
| Notch2 | IHC | 1:50 | Cell Signaling Technology | 5732 |
| JAGGED1 | IHC | 1:100 | Cell Signaling Technology | 70109 |
| Phospho-AKTSer473 | IHC | 1:250 | Cell Signaling Technology | 4060 |
| Phospho-GSK-3βSer9 | IHC | 1:200 | Cell Signaling Technology | 9323 |
| Phospho-RPS6Ser235/236 | IHC | 1:400 | Cell Signaling Technology | 4856 |
| FOXA1 | IHC | 1:400 | Abcam | ab170933 |
| FOXA2 | IHC | 1:200 | Abcam | ab108422 |
| CEBPA | IHC | 1:100 | Cell Signaling Technology | 8178 |
| CPS1 | IHC | 1:200 | Abcam | ab129076 |
| CYP2E1 | IHC | 1:200 | Proteintech | 19937-I-AP |
| CYP3A4 | IHC | 1:400 | Proteintech | 18227-1-AP |
| ARG1 | IHC | 1:500 | Abcam | ab233548 |
| GLUL | IHC | 1:500 | BD Biosciences | 610517 |
| CD44v6 | IHC | 1:100 | ThermoFischer Scientific | MA1-81995 |
| CD133 | IHC | 1:100 | Abcam | ab271092 |
| EPCAM | IHC | 1:100 | Abcam | ab221552 |
| CD34 | IHC | 1:150 | Abcam | Ab8158 |
| Podoplanin | IHC | 1:200 | Abcam | ab256559 |
| NCAM1/CD56 | IHC | 1:400 | Cell Signaling Technology | 99746 |
| Ki67 | IHC | 1:500 | Bethyl Laboratories | IHC-00375 |
| S100A4 | IHC | 1:100 | Cell Signaling Technology | 13018 |
| PDGFRβ | IHC | 1:100 | Cell Signaling Technology | 4564 |
| Phospho-LATS1/2T1041/1079 | IHC | 1:100 | Abcam | 11344 |
| Cleaved Caspase 3 | IHC | 1:50 | Cell Signaling Technology | 9661 |
| Cleaved PARP | IHC | 1:100 | Cell Signaling Technology | 94885 |
| CD4 | IHC | 1:100 | Cell Signaling Technology | 25229 |
| CD45 | IHC | 1:100 | Cell Signaling Technology | 70257 |
| F4/80 | IHC | 1:200 | Cell Signaling Technology | 70076 |
| β-Actin | WB | 1:5000 | Santa Cruz Biotechnology | sc-10731 |