FIG. 1.

Southern blot detection of the genomic fragment harboring the Tn916ΔE insertion in HLT2. Genomic DNAs from HLT2 (lane 1), the wild-type parental strain (lane 2), and HLT2 transductant HTL2/1 (lane 3) were digested with EcoRI and hybridized with a digoxigenin-labeled SSP-PCR fragment flanking the transposon. Lane M contained lambda DNA digested with HindIII, which was labeled with digoxigenin and used as markers in the same blot (the fragment sizes are, from top to bottom, 23, 9.4, 6.5, 4.3, 2.3, and 2.0 kb).