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Mechanism of SPIO-induced macrophage autophagy. (A) Western blotting of LC3-II and p62 in Raw264.7 treated with SPIO nanoparticles for 24 h with or without adding CLI-095, β-actin was detected as a loading control. Densitometry analysis for quantifying the levels of LC3-II (B) and p62 (C). (D) Expression of p62 mRNA in Raw264.7 treated with SPIO nanoparticles for 24 h detected by RT-qPCR. Values are expressed as the mean, n = 3. Bars show SD. **P < 0.01; ***P < 0.001
