TABLE 1.

Stability and copy number in pGM-IL, pGMA-IL, and pGM-GAM transformants^a

Vector	Strain	Carbon source	Stability of integrated sequencesb (%)	Vector stabilityc (%)	Copy number increased
pGM-IL	BT16	Galactose	99 ± 1.5	95 ± 4.4	6.6 ± 1.6
		Glucose	100 ± 0.0	100 ± 0.0
pGMA-IL	BT16	Galactose	99 ± 1.9	78 ± 6.4	11 ± 3.3
		Glucose	100 ± 0.0	89 ± 6.2
pGM-GAM	BT16	Galactose	100 ± 0.0	97 ± 2.5	6.7 ± 1.4
		Glucose	100 ± 0.0	100 ± 0.0
pGM-IL	MW98-8C	Galactose		99 ± 1	1.4 ± 0.4
		Glucose		99 ± 1
pGMA-IL	MW98-8C	Galactose		98 ± 1.7	1.5 ± 0.2
		Glucose		96 ± 4.3

^aResults are means and standard deviations for four independent transformants. 

^bPercentage of Ura⁺ cells after 24 h of growth on the indicated carbon source. 

^cPercentage of G418^r cells after 24 h of growth on the indicated carbon source. 

^dRelative increase (YPG/YPD) of vector copy number per G418^r cell.